| Variable | Control | Met-Lys | EE | P< |
| Chemical composition of the diet | ||||
| Crude Protein, % total DMI | 17.8b | 18.4a | 0.07 | <0.001 |
| NDF, % total DMI | 42.7 | 42.3 | 1.80 | 0.66 |
| EE, % total DMI | 3.43b | 3.83a | 0.07 | 0.004 |
| TDN, % total DMI | 66.5 | 67.3 | 1.86 | 0.39 |
| NEL, Mcal/kg total DMI | 1.49 | 1.50 | 0.02 | 0.60 |
| DMI, kg cow−1 day−1 | ||||
| Pasture | 11.5 | 11.9 | 2.67 | 0.58 |
| Concentrate | 7.58 | 7.56 | 0.57 | 0.97 |
| Total | 19.1 | 19.6 | 1.30 | 0.69 |
| Protein Intake and Amino Acid Supply | ||||
| Protein, g DM cow−1 day−1 | 3476 | 3525 | 233 | 0.84 |
| Met supply, g DM cow−1 day−1 | 79.7b | 165ª | 7.47 | 0.006 |
| Lys supply, g DM cow−1 day−1 | 193b | 264ª | 15.6 | 0.006 |