Name

Primer sequence (5’ to 3’)

Purpose

Annealing temperatures/˚C

6-F

AAGCTCACACCAACAAAGGGG

Cloning of the Middle Fragment

63.6

6-R

TCAAGGGTCTGTGAAATCTGT

57.2

6-3’-F

TGTCAGTGCACAAATGGCTTC

3’RACE PCR

62.2

B26

GACTCGAGTCGACATCGATTTTTTTTTTTTTTTTT

70.4

RrMYB6-F

ATGGGAAGGTCACCTTGCTGT

ORF PCR

63.1

RrMYB6-R

CTATGAATTCAAGGGTCTGTG

54.8

Actin-F

CACTTAGCACCTTCCAGCAGATGT

Real-time Quantitative PCR

64.0

Actin-R

CTACAACAGCAGACCTGAGTTCACT

61.3

RrMYB6-Q-F

CACCGAAGAAGAAGACGAG

56.1

RrMYB6-Q-R

TTCATTGTCGGTTCGTCC

57.9