Name | Primer sequence (5’ to 3’) | Purpose | Annealing temperatures/˚C |
6-F | AAGCTCACACCAACAAAGGGG | Cloning of the Middle Fragment | 63.6 |
6-R | TCAAGGGTCTGTGAAATCTGT | 57.2 | |
6-3’-F | TGTCAGTGCACAAATGGCTTC | 3’RACE PCR | 62.2 |
B26 | GACTCGAGTCGACATCGATTTTTTTTTTTTTTTTT | 70.4 | |
RrMYB6-F | ATGGGAAGGTCACCTTGCTGT | ORF PCR | 63.1 |
RrMYB6-R | CTATGAATTCAAGGGTCTGTG | 54.8 | |
Actin-F | CACTTAGCACCTTCCAGCAGATGT | Real-time Quantitative PCR | 64.0 |
Actin-R | CTACAACAGCAGACCTGAGTTCACT | 61.3 | |
RrMYB6-Q-F | CACCGAAGAAGAAGACGAG | 56.1 | |
RrMYB6-Q-R | TTCATTGTCGGTTCGTCC | 57.9 |