##

Position/Strand

Target motif

MIT Specificity Score

CFD Spec. score

Predicted Efficiency,

Doench’16

Predicted Efficiency,

Mor.-Mateos

Outcome

Out-of-Frame

Off-targets for

0-1-2-3-4 mismatches

1

32/fw

GCTCGTTGCCCTCGCTCTCC TGG

94

97

37

22

53

0-0-0-7-37

44 off-targets

2

41/fw

CCTCGCTCTCCTGGCTCTCG CGG

89

93

57

42

58

0-0-1-15-94

110 off-targets

3

30/rev

CGGCGCTCGCCGCGAGAGCC AGG

(high GC content)

84

83

32

59

53

0-0-0-8-125

133 off-targets

4

20/rev

CGCGAGAGCCAGGAGAGCGA GGG

77

88

54

51

40

0-0-0-14-151

165 off-targets

5

21/rev

CCGCGAGAGCCAGGAGAGCG AGG

59

80

49

59

41

0-0-2-9-259

270 off-targets