##

Position/Strand

Target motif

MIT Specificity Score

CFD Spec. score

Predicted Efficiency,

Doench’16

Predicted Efficiency,

Mor.-Mateos

Outcome

Out-of-Frame

Off-targets for

0-1-2-3-4 mismatches

1

8/rev

CGCAAGGAGGACAAATATCT TGG

17

86

39

39

61

3-2-9-6-89

109 off-targets

2

33/rev

TTGTGCTCACTGAAAGAGCA AGG

9

86

61

51

64

8-2-5-4-27

46 off-targets

3

21/rev

AAAGAGCAAGGAGCGCAAGG AGG

8

72

67

57

46

11-4-3-16-131

165 off-targets

4

24/rev

CTGAAAGAGCAAGGAGCGCA AGG

7

86

51

64

43

10-4-3-3-27

47 off-targets