Combination of amino acid

Concentration (mg/L)

Average number of SSE per primary SE

Average number of clusters per primary SE

Average number of SSE per cluster

Control medium

0

4.53 ± 0.57 abcde

0.6 ± 0.11 a

1.73 ± 0.39 abc

COMB1:

Glutamine + Asparagine + Arginine

10 + 10 + 10

3.33 ± 0.42 def

0.36 ± 0.11 abcd

1.46 ± 0.51 abcde

30 + 30 + 30

3.63 ± 0.39 cde

0.23 ± 0.07 bcd

0.57 ± 0.22 ef

500 + 500 + 500

4.36 ± 0.48 bcde

0.36 ± 0.08 abcd

0.8 ± 0.25 cdef

COMB2:

Glutamine + Asparagine + Arginine + Methionine + Tryptophan

10 + 10 + 10 + 10 + 10

4.43 ± 0.39 abcde

0.6 ± 0.09 a

1.53 ± 0.28 abcde

30 + 30 + 30 + 30 + 30

5.00 ± 0.46 abcd

0.4 ± 0.09 abc

1.06 ± 0.28 bcdef

500 + 500 + 500 + 500 + 500

0.9 ± 0.18 g

0.4 ± 0.09 abc

0.9 ± 0.25 bcdef