| Primer | Sequence (5¢®3¢) | Tm (˚C) | Purpose | |
| sigVA-F | CATTATTGCGTACGGAGAC | 58.1 | For marker-free deletion of sigV | |
| sigVA-R | TGCAATAAAGGGCTCCT | 58.3 |
| |
| sigVB-F2 | CAAAAAGGAGCCCTTTATTGCAATGGATAAGAGATTACAGC | 76.8 |
| |
| sigVB-R | GCTTGAGTCAATTCCGCTGTCGGCTTTGTGTGTAGGAAG | 82.5 |
| |
| sigVC-F | CAAAATTAACGTACTGATTGGGTAGGATCCGCGCAGGTTGGCTTTCAGTTATG | 84.8 |
| |
| sigVC-R | CGTGTTTTCTCCTGTAATTTCC | 61.2 |
| |
| rsiVA-F | CTAGGTAACAGCCTACG | 50.2 | For marker-free deletion of rsiV | |
| rsiVA-R | TAAGAAAGATCCTCCTTCG | 56.0 |
| |
| rsiVB-F | GTTGACGAAGGAGGATCTTTCTTAATCCATCTAAGATTATGGATG | 76.4 |
| |
| rsiVBmazF-R | GCTTGAGTCAATTCCGCTGTCGGTTCTTCAATCGCCAGCGAC | 87.6 |
| |
| mazFrsiVC-F | CAAAATTAACGTACTGATTGGGTAGGATC | 87.6 |
| |
| rsiVC-R | GATAGAGAGTAAATCTGGCGTGTC | 61.9 |
| |
| pAPNC-F | CGACAGCGGAATTGACTCAAGC | 70.0 |
| |
| chpA-R | CGCGGATCCTACCCAATCAGTACGTTAATTTTG | 75.7 |
| |
| sigVcheck-F | CACCTTTAACAGGCTATGCC | 61.0 | For confirmation of marker-free deletion of sigV | |
| rsiVcheck-F | CCTTCATGATATGAGCAG | 53.7 | For confirmation of marker-free deletion of rsiV | |
| rsiVcheck-R | CACAAGCAATGATATCAC | 51.6 | For confirmation of marker-free deletion of sigV and rsiV | |
| RsiVNup | GGAGATTCCTAGGATGGACTACAAAGACGACGACGACAAAATGGATAAGAGATTACAGCAATTAAGAG | 78.1 | For construction of chimeric anti-σ factors RsiVN-RsiWTMC and RsiVNTM-RsiWC | |
| RsiVNdown | GGATGGGTTCTGAACCATTTCTTTTTTGGTTCTTGCTG | 79.2 | For construction of chimeric anti-σ factor RsiVN-RsiWTMC | |
| RsiVNTMdown | GTCATTATGCCAGCTGTTGATATTAACAAGCGCAGTG | 77.4 | For construction of chimeric anti-σ factor RsiVNTM-RsiWC | |
| RsiWTMCup | TGGTTCAGAACCCATCCCGTTATCG | 73.7 | For construction of chimeric anti-σ factor RsiVN-RsiWTMC | |
| RsiWTMCdown | TCGAGGGGGGGCCCGTGTTACTCTTCTCCGTTCGGAT | 87.2 | For construction of chimeric anti-σ factors RsiVN-RsiWTMC and RsiVNTM-RsiWC | |
| RsiWCup | AACAGCTGGCATAATGA | 60.1 | For construction of chimeric anti-σ factor RsiVNTM-RsiWC | |
| pAPNC-SalI-up | CTGCAGGCATGCCTGCAGGGAGATTCCTAGGATGGACTAC | 84.4 | For construction of chimeric anti-σ factors | |
| pAPNC-SalI-down | CCGGGGATCCTCTAGAGTCGAGGGGGGGCCCGTG | 88.6 |
| |
| pAPNCcheckF | ACAAGGTGTGGCATAATGTG | 60.9 | For sequence confirmation of chimeric anti-σ factors | |
| pAPNCcheckR | GCTATATTGGCCGCTTCCTG | 65.5 |
| |