Strain | Relevant genotype | Construction, source or reference |
E. coli |
|
|
DH5 | recA1 endA1 hsdR17 | Laboratory collection |
B. subtilis |
|
|
168 | trpC2 | Laboratory collection |
YTB019 | 168 ∆ugtP::kan | [34] |
YTB040 | YTB019 amyE::(PsigV-lacZ cat) | [14] |
YTB041 | 168 amyE::(PsigV-lacZ cat) | [14] |
1012 rasP | leuA8 metB5 trpC2 hsrM1 rasP::tet | [30] [35] |
SBS100 | 168 rasP::tet | 1012 rasP ®a 168 |
SBS101 | YTB041 rasP::tet | SBS100 ® YTB041 |
SBS102 | YTB040 rasP::tet | SBS100 ® YTB040 |
SBS200 | 168 rsiV::(pMUTIN-Pspac-flag-rsiV erm) | K. Asai |
SBS201 | SBS200 ∆ugtP::kan | YTB019 ® SBS200 |
SBS204 | SBS200 amyE::(PsigV-lacZ cat) | YTB041 ® SBS200 |
SBS205 | SBS204 ∆ugtP::kan | YTB019 ® SBS204 |
TMO310 | 168 aprE::(lacI Pspac-mazF spc) | [21] |
SBS303 | 168 sigV::(lacI Pspac-mazF spc) | This study |
SBS304 | 168 ∆sigV | This study |
SBS313 | SBS304 amyE::(PsigV-lacZ cat) | YTB041 ® SBS304 |
SBS314 | SBS313 ∆ugtP::kan | YTB019 ® SBS313 |
SBS351 | 168 rsiV::(lacI Pspac-mazF spc) | This study |
SBS352 | 168 ∆rsiV | This study |
SBS361 | SBS352 amyE::(PsigV-lacZ cat) | YTB041 ® SBS352 |
SBS362 | SBS361 ∆ugtP::kan | YTB019 ® SBS361 |
SBS500 | SBS361 aprE::(lacI Pspac-RBS-rsiVN-rsiWTMC spc) | pAP-RsiVN-RsiWTMC ® SBS361 |
SBS501 | SBS500 ∆ugtP::kan | YTB019 ® SBS500 |
SBS502 | SBS361 aprE::(lacI Pspac-RBS-rsiVNTM-rsiWC spc) | pAP-RsiVNTM-RsiWC ® SBS361 |
SBS503 | SBS502 ∆ugtP::kan | YTB019 ® SBS502 |