Gene name

Primer

Thermo Tm

fdhE

F: ACCAAGAAGCTCGACTACC

R: GCCAATTCGAGATAGAGCTG

54.5˚C

57.7˚C

metE

F: TTCGATACCAACTACCACTATCTG

R: GATCTGTACCCACTCGACTC

57.4˚C

54.5˚C

pilK

F: CTGTTGATCTACTTCCGCC

R: GAACCTGATCATTGGCAACC

55.8˚C

60.3˚C

rpoN

F: TGGCTGGAGAAGAAGTATTTGAG

R: AACGAAACACTGATGAAGGTC

55.0˚C

55.0˚C