| Aminoglycosides | Precursor ion (m/z) | Cone voltage (V) | Quantification ion (m/z) | Collision energy (V) | Confirmation ion (m/z) | Collision energy (V) | |
| Apramycin | 540.3 | 90 | 217 | 25 | 378 | 15 | |
| Gentamycin | 478.3 464.3 450.1 | 120
| 322.2 322.1 322 | 10
| 156.9
| 20
| |
| Dihydrostreptomycin | 584.3 | 120 | 262.9 | 30 | 245.9 | 35 | |
| Streptomycin | 582.2 | 120 | 263.1 | 35 | 245.9 | 35 | |
| Néomycin | 615.3 | 120 | 160.9 | 35 | 292.9 | 25 | |
| Spectinomycin | 351.1 | 120 | 333.1 | 15 | 207.1 | 20 | |
| Paromomycin | 616.3 | 100 | 162.9 | 40 | 324.1 | 20 | |
| Tobramycin | 468.2 | 120 | 162.9 | 20 | 324.2 | 10 | |
| Amikacin | 586.3 | 110 | 162.9 | 35 | 425.3 | 15 | |
| Sisomycin | 448.3 | 100 | 254.1 | 15 | 271.1 | 15 | |
| Kanamycin | 485 | 60 | 324.2 | 15 | 163.2 | 5 | |
| Hygromycin | 528.2 | 110 | 177 | 30 | 352.1 | 25 | |