Test Protocol

Metagenomic Shotgun Sequencing

EPA Approved Traditional

Culturing Techniques

Expected Results

Levels and Diversity of Traditional Indicator

Bacteria, Pathogenic Bacteria, Virulent

Genes, Antibiotic Resistant Genes

Levels of Fecal Colifom Bacteria, Enterococci spp., Possible Contamination

Sample ID

Traditional Indicators (genomic reads/100ml)

Known Pathogens

(genomic reads/100ml)

Total Number of Virulence Genes

Total Number of

Antibiotic Resistance Genes

Fecal coliform (CFU/100ml)

Enterococci spp. (CFU/100ml)

ABI1004

(Storm Water Drain)

499

2600

14

1

>2000

230

ABI1003

(Storm Water Drain)

24182

58700

289

70

>2000

>2000

ABI1002

(Freshwater Collection Pond)

3651

4750

6

1

220

44

ABI1001

(Equiptment Blank/Control)

0

0

0

0

0

0