| Technique |
Kit |
Manufacturer |
Estimated cost |
Authors |
| miRNA isolation |
Ambion mirVana miRNA isolation kit |
Life technologies (ThermoFisher Scientific) |
USD 384.00 |
Xie et al., 2012 [20] ; Hou et al., 2014 [44] ; Villegas-Ruiz et al., 2014 [25] ; Ribeiro et al., 2015 [49] ; Zhang et al., 2016 [45] ; Shishodia et al., 2015 [38] ; Luo et al., 2015 [53] ; Wei et al., 2017 [18] |
| Recover all total nucleic acid isolation kit from applied biosystems |
Ambion (ThermoFisher Scientific) |
USD 374.00 |
Deftereos et al., 2011 [27] |
|
| miRNeasy FFPE kit |
Qiagen |
USD 474.00 |
Zheng et al., 2015 [30] |
|
| RNeasy FFPE kit |
Qiagen |
USD 484.00 |
Ma et al., 2015 [32] |
|
InvitrogenTM TRIzolTM Reagent |
Invitrogen (ThermoFisher Scientific) |
USD 209-374.00 |
Gozce et al.(2015) [26] ; Wen et al., 2017 [22] ; Jiminez-Wences et al., (2016) [37] ; Wang et al., 2014 [35] ; Burmrungthai et al., 2016 [28] ; Cheung et al., 2012 [36] ; Ye et al., 2019 [48] ; Han et al., 2015 [17] ; Huang et al., 2016 [24] ; Zhang et al., 2017 [41] ; Shen et al. 2013 [52] ; Leitao et al., 2014 [42] ; Babion et al. 2018 [43] |
|
Mirneasy mini kit |
Qiagen |
USD 402.00 |
Kawai et al., 2017 [51] ; Xin et al., 2016 [47] |
|
High pure miRNA isolation kit |
Roche |
USD 504.50 |
Lukic et al., 2018 [33] |
|
Invitrogen mirVANA miRNA isolation kit |
ThermoFisher Scientific |
USD 326.00 |
Luo et al., 2015 [53] |
|
Total RNA isolation kit |
Beijing Tiandz gene technology |
N\A |
Zhang et al., 2019 [54] |
|
| Reverse Transcription |
TaqMan MicroRNA reverse transcription kit |
Applied biosystems (ThermoFisher Scientific) |
USD 397-1014.00 |
Xie et al., 2012 [20] ; Han et al. 2015 [17] ; Ribeiro et al., 2015 [49] ; Malta et al., 2015 [50] ; Jiminez-Wences et al., 2016 [37] ; Deftereos et al., 2011 [27] ; Cheung et al., 2012 [36] ; Wang et al., 2014 [35] , Kawai et al. [51] , Jiang et al., 2017 [46] ; Babion et al., 2018 [43] ; Wei et al., 2017 [18] |
| High capacity cDNA reverse transcription kit |
Applied biosystems (ThermoFisher Scientific) |
USD 353-1360.00 |
Zhang et al., 2016 [45] |
|
| MiraMasTM kit |
Bioo scientific |
N\A |
Chen et al., 2013 [39] |
|
| M-MLV kit |
Promega |
USD 25-364.00 |
Zhang et al., 2013 [21] ; Luo et al., 2015 [53] |
|
| PrimeScript RT reagent kit |
Takara Bio |
N/A |
Wen et al., 2017 [22] |
|
|
|
MirCute miRNAs kit |
Tiangen |
USD 652.00 |
Han et al., 2015 [17] |
| Quant reverse transcriptase |
|
N\A |
Zeng et al., 2015 [29] |
|
| Takara reverse transcription kit |
Takara Bio |
N/A |
Huang et al., 2016 [24] |
|
| SuperScriptTM III reverse transcriptase kit |
Invitrogen |
USD 111-1368.00 |
Zhang et al., 2017 [41] |
|
| Brilliant II SYBR Green QRT-PCR, AffinityScript two-step |
Agilent technologies |
USD 849.00 |
Lukic et al., 2018 [33] |
|
| Reverse transcriptase kit |
Hangzhou Bioer technology |
N\A |
Zhang et al., 2019 [54] |
|
| Real-Time PCR |
TaqMan MicroRNA assays |
Applied biosystems (ThermoFisher Scientific) |
USD 313-2616.00 |
Xie et al., 2012 [20] ; Han et al., 2015 [17] ; Ribeiro et al. 2015 [49] ; Malta et al., 2015 [50] ; Jiminez-Wences et al., 2016 [37] ; Deftereos et al., 2011 [27] ; Cheung et al., 2012 [36] ; Wang et al., 2014 [35] , Bierkens et al., 2012 [31] ; Zhang et al., 2017 [41] ; Kawai et al. [51] ; Lukic et al., 2018 [33] ; Shen et al., 2013 [52] ; Jiang et al., 2017 [46] ; Babion et al., 2018 [43] ; Wei et al., 2017 [18] |
| SYBR Green MIX kit |
Takara Bio |
N\A |
Wen et al., 2017 [22] |
|
| SYBR Premix Ex Taq II |
Takara Bio |
N\A |
Huang et al., 2016 [24] |
|
| SYBR Green specific primers |
Takara Bio |
N/A |
Li et al., 2011 [23] |
|
| SYBR Green real-time PCR universal reagent |
GenePharma Co. Ltd. |
N/A |
Yao and Lin, 2012 [34] |
|
| Fluorescent quantitative detection kit specific primers |
Shanghai biological engineering and Guangzhou ruibo |
N/A |
Han et al., 2015 [17] |
|
| miScript miRNA detection system |
Qiagen |
N\A (discontinued) |
Zhang et al., 2016 [45] ; Xin et al., 2016 [47] |
|
| mirVANA qRT-PCR kit |
Applied biosystems (ThermoFisher Scientific) |
N\A (discontinued) |
Shisodia et al., 2015 [38] |
|
| SuperReal PreMix plus (SYBR green) |
Tiangen |
USD 2830.90 |
Zeng et al., 2015 [29] |
|
| SYBRTM Green PCR master mix |
Applied biosystems |
USD 127-3880.00 |
Ma et al., 2015 [32] |
|
| Hairpin-itTM miRNA qPCR quantitation kit |
Genepharma |
N\A |
Luo et al., 2015 [53] |
|
| QuantiTect SYBR Green PCR kit |
Qiagen |
USD 473-1946.00 |
Leitao et al., 2014 [47] |
|
| RT-PCR mixture |
Bio-Rad |
N\A |
Zhang et al., 2019 [54] |