LSUF1/R1

GSP7(3’ RACE)

ITSF/ITSR

F/R

IGSF1/R1

① 95˚C 3 min

① 94˚C 30 s

① 94˚C 3 min

① 95˚C 3 min

① 95˚C 3 min

② 94˚C 30 s

② 68˚C 30 s

② 94˚C 30 s

② 94˚C 30 s

② 94˚C 30 s

③ 48˚C 30 s

③ 72˚C 5 min

③ 53˚C 30 s

③ 55˚C 30 s

③ 56˚C 30 s

④ 72˚C 1 min

④ ①-③ 20 cycles

④ 72˚C 1.5 min

④ 72˚C 5 min

④ 72˚C 1.5 min

⑤ ②-④ 35 cycles

⑤ ②-④ 35 cycles

⑤ ②-④ 35 cycles

⑤ ②-④ 35 cycles

⑥ 72˚C 7 min

⑥ 72˚C 10 min

⑥ 72˚C 7 min

⑥ 72˚C 7 min