LSUF1/R1 | GSP7(3’ RACE) | ITSF/ITSR | F/R | IGSF1/R1 |
① 95˚C 3 min | ① 94˚C 30 s | ① 94˚C 3 min | ① 95˚C 3 min | ① 95˚C 3 min |
② 94˚C 30 s | ② 68˚C 30 s | ② 94˚C 30 s | ② 94˚C 30 s | ② 94˚C 30 s |
③ 48˚C 30 s | ③ 72˚C 5 min | ③ 53˚C 30 s | ③ 55˚C 30 s | ③ 56˚C 30 s |
④ 72˚C 1 min | ④ ①-③ 20 cycles | ④ 72˚C 1.5 min | ④ 72˚C 5 min | ④ 72˚C 1.5 min |
⑤ ②-④ 35 cycles | ⑤ ②-④ 35 cycles | ⑤ ②-④ 35 cycles | ⑤ ②-④ 35 cycles | |
⑥ 72˚C 7 min | ⑥ 72˚C 10 min | ⑥ 72˚C 7 min | ⑥ 72˚C 7 min |