| S.N | Reagent/Sample | Blank | Standard | Test |
| 1 | Urine |
|
| 1 ml |
| 2 | Standard |
| 1 ml |
|
| 3 | Distilled Water | 1 ml |
|
|
| 4 | CuSO4 (0.01) M | 1 ml | 1 ml | 1 ml |
| 5 | NaOH (2.5) M | 1 ml | 1 ml | 1 ml |
| 6 | 6.6% H2O2 | 1 ml | 1 ml | 1 ml |
| 7 | 3N H2SO4 (with agitation) | 4 ml | 4 ml | 4 ml |
| 8 | 5% P-dimethylamionbenzaldehyde | 2 ml | 2 ml | 2 ml |