| Experiment | Five | |
| Parameters | Treatment | |
| T (˚C) | 35 | ZM |
| Light (Lux) | 5000 | FW |
| Period (days) | 21 | FW-BW |
| Rep. No. | 4 | FW-MW |
| Volume/Replicate | 250 mL | FW-PAS 3% |
| Spirulina: medium | 1:1 | FW-IMJ-1 |
| 9:1 | FW-MIX | |