| TC Method | SF Method | |||||||
Time (min) | % A | % B | % C | Flow rate (mL∙min−1) | Time (min) | % A | % B | % C | Flow rate (mL∙min−1) |
1 | 80 | 5 | 15 | 0.15 | 1 | 80 | 5 | 15 | 015 |
15 | 60 | 25 | 15 | 0.15 | 15 | 60 | 25 | 15 | 0.15 |
16 | 5 | 5 | 90 | 0.15 | 16 | 5 | 5 | 90 | 0.25 |
26 | 5 | 5 | 90 | 0.15 | 20 | 5 | 5 | 90 | 0.25 |
27 | 80 | 5 | 15 | 0.15 | 26 | 5 | 5 | 90 | 0.15 |
35 | 80 | 5 | 15 | 0.15 | 27 | 15 | 5 | 15 | 0.15 |
- | - | - | - | - | 35 | 15 | 5 | 15 | 0.15 |
Column temperature | 15˚C | 25˚C | |||||||
Injection Volume | 10 μL | 20 L |