Primer name | (5’→3’) Nucleotide sequence* | Purpose |
β-F | ATGGGTTCCGAAGGCCTC | Intermediate segment amplification |
β-R | GAACAATGTGCTTCCATCTGC | |
B26 | GACTCGAGTCGACATCGATTTTTTTTTTTTTTTTT | 3’RACE PCR for RrG-beta1 |
β-3’-F | GCAGATGGAAGCACATTGTTC | |
RrG-beta1-F | ATGGGTTCCGAAGGCCTC | Full-length cDNA forRrG-beta1 |
RrG-beta1 | GGGGAATTGGCCGCTTCTAG | |
Actin-F | CACTTAGCACCTTCCAGCAGATGT | qRT-PCR forRrG-beta1 and Actin |
Actin-R | CTACAACAGCAGACCTGAGTTCACT | |
RrG-beta1-Q-F | ATGAGGGCCCACACCGAC | |
RrG-beta1-Q-R | TGAGCTGCCAGAGGATGATG | Expression vector constructionforRrG-beta1 |
RrG-beta1-S-F | ACTAGTATGGGTTCCGAAGGCCTC | |
RrG-beta1-P-R | CACGTGCTAGAAGCGGCCAATTCCCC |