SI. No. | Type of Explant | PGRs | Response% | Callus intensity | |||
PGRs | µM | PGRs | µM | ||||
1. | Leaf segment | 2,4-D | 0.86 |
|
| 00 | -- |
2. | 2,4-D | 2.1 |
|
| 00 | -- | |
3. | 2,4-D | 2.1 | BAP | 0.49 | 00 | 00 | |
4. | Leaf sheath | 2,4-D | 0.86 |
|
| 36.67 | + |
5. | 2,4-D | 2.1 |
|
| 66.67 | +++ | |
6. | 2,4-D | 2.1 | BAP | 0.49 | 53.33 | ++ | |
7. | Shoot tip | 2,4-D | 0.86 |
|
| 33.33 | + |
8. | 2,4-D | 2.1 |
|
| 46.67 | ++ | |
9. | 2,4-D | 2.1 | BAP | 0.49 | 36.67 | + | |
10. | Nodal segments | 2,4-D | 0.86 |
|
| 88.33 | ++++ |
11. | 2,4-D | 2.1 |
|
| 100.00 | +++++ | |
12. | 2,4-D | 2.1 | BAP | 0.49 | 98.33 | +++++ | |
13. | Internode segments | 2,4-D | 0.86 |
|
| 00 | -- |
14. | 2,4-D | 2.1 |
|
| 16.67 | + | |
15. | 2,4-D | 2.1 | BAP | 0.49 | 00 | --- | |
SE |
|
|
|
| 26.77 |
| |
CD |
|
|
|
| 54.66 |
|