V2

A-U in position 1; U-A in position 5

V3

G:Y in position 4; A A or C A bulges in position 8.

BDV-j (3)

V1

G:Y in position 3 (exception A-U); G:Y in position 9; G:Y in position 14 (exception A-U); U-A or Y:G in position 16; C C, U C bulges, U-A or Y:G in position 19

V2

A-U in position 1; U-A in position 5

V3

G:Y in position 4; Y:G, G:Y, U C or A A bulges in position 8

BDV sub-genotypes

Locus

Characteristic PNS markers

BDV-a1 (1)

V1

U right nucleotide in position 7; A-U in position 12; A-U or CU in position 15; AA or AG in position 18

BDV-a2 (1)

V1

G right nucleotide in position 7; G:Y or CC in posizion 12; G:Y in position 15; AG or GG in position 18

BDV-a3 (1)

V1

A right nucleotide in position 7; G:Y in position 12; AC in position 15; AG in position 18

BDV-a4 (1)

V1

G right nucleotide in position 7; G:Y in posizion 12; G:Y in position 15; A-U in position 18

BDV-c1.1 (Sw)

V1

A-U in position 16

V2

A-U in position 1

V3

G:Y in position 4

BDV-c1.2 (8)

V1

A-U in position 16

V2

A-U in position 1

V3

A-U in position 4

BDV-c1.3 (8)

V1

A C bulge in position 16; G:Y in position 21

V2

A-U in position 1

V3

G:Y in position 4

BDV-c2 (6)

V1

G:Y or U C and U U bulges in position 20; U or U U bulge in position 21

V2

A C bulge in position 1

V3

C C bulge in position 7 (exception U C bulge)

BDV-d1 (3)

V1

CU or UU in position 19

V3

A-U in position 4; A G bulge in position 8

BDV-d2 (3)

V1

CA in position 19

V3

G:Y in position 4; A A bulge in position 8

BDV-e1 (4)

V1

G:Y in position 16

V3

U-A in position 8

BDV-e2 (4)

V1

A-U in position 16; G in position 21

V3

CA in position 8

BDV-f1 (4, 5)

V1

G right nucleotide in position 7; CU in position 17; GG in position 18

V2

G:Y or Y:G in position 2

V3

UU or UC in position 7

BDV-f2 (4)

V1

U right nucleotide in position 7; Y:G in position 17; G:Y in position 18

V2

UA in position 2

V3

A-U or AC in position 7