Stationary phase | Phenomenex Lux Cellulose-4 column (250 × 4.6 mm; 5 µ particle size) |
Mobile phase | Isopropanol:n-Heptane (60:40) |
Flow rate | 1 ml/min |
Injection volume | 20 µL |
Column temperature | 40˚C |
Detection wavelength | 254 nm |