Fragment

Predenature

Denature

Annealing

Extension

Cycles

Extension

Size (bp)

Exon 2-1

94˚C, 2 min

94˚C, 15 s

64˚C, 30 s

68˚C, 18 s

35

68˚C, 4 min

274

Exon 2-2

94˚C, 2 min

94˚C, 15 s

60˚C, 30 s

68˚C, 14 s

35

68˚C, 4 min

206

Exon 3

94˚C, 2 min

94˚C, 15 s

60˚C, 30 s

68˚C, 21 s

35

68˚C, 4 min

258

Exon 4-1

94˚C, 2 min

94˚C, 15 s

68˚C, 30 s

68˚C, 18 s

35

68˚C, 4 min

271

Exon 4-2

94˚C, 2 min

94˚C, 15 s

60˚C, 30 s

68˚C, 21 s

35

68˚C, 4 min

243

Exon 5

94˚C, 2 min

94˚C, 15 s

60˚C, 30 s

68˚C, 21 s

35

68˚C, 4 min

291

Exon 6

94˚C, 2 min

94˚C, 15 s

66˚C, 30 s

68˚C, 21 s

35

68˚C, 4 min

311

Exon 7

94˚C, 2 min

94˚C, 15 s

58˚C, 30 s

68˚C, 21 s

35

68˚C, 4 min

350

Exon 8

94˚C, 2 min

94˚C, 15 s

60˚C, 30 s

68˚C, 21 s

35

68˚C, 4 min

291

Exon 9

94˚C, 2 min

94˚C, 15 s

60˚C, 30 s

68˚C, 18 s

35

68˚C, 4 min

289

Exon 10

94˚C, 2 min

94˚C, 15 s

68˚C, 30 s

68˚C, 16 s

35

68˚C, 4 min

254

Exon 11

94˚C, 2 min

94˚C, 15 s

60˚C, 30 s

68˚C, 18 s

35

68˚C, 4 min

284

Exon 12

94˚C, 2 min

94˚C, 15 s

60˚C, 30 s

68˚C, 21 s

35

68˚C, 4 min

233

Exon 13

94˚C, 2 min

94˚C, 15 s

68˚C, 30 s

68˚C, 16 s

35

68˚C, 4 min

259

Exon 14

94˚C, 2 min

94˚C, 15 s

66˚C, 30 s

68˚C, 16 s

35

68˚C, 4 min

251