Fragment | Forward primer (5' → 3') | Tm (˚C) | Reverse primer (5' → 3') | Tm (˚C) |
Exon 2-1a | CTGAAGACCAAGAGGGAAGA | 52 | CAATGACGGACAGGAGAAAG | 52 |
Exon 2-2a | CAAGGAGCAGAACGGAGTG | 53 | GGAGGCTGAGATGGGACTT | 53 |
Exon 3 | TCCGAGGCCCCAAACTAAA | 51 | ATGATGCGGCTGGCTTGCT | 53 |
Exon 4-1a | TGATGGTGGCTCTGTGCTC | 53 | GTGGTCCCAAAAGTGTCGT | 51 |
Exon 4-2a | GGGCGCTGCACTATCTCTT | 53 | TCCAACCAAGGGGCTACCA | 53 |
Exon 5 | TTGACAGCCACCCACAGAGT | 54 | AGCACAAAACCCAACTGAGG | 52 |
Exon 6 | GCGTCCCAGGAAATGTTTG | 51 | CCCTGTGGACTGTGAAGCA | 53 |
Exon 7 | GCATCTTCCACCAGTCGTCT | 54 | TGTTCTCATCCAGGGACACC | 54 |
Exon 8 | CCTTCCCCAGACACAGTAA | 51 | AAAAAGGCTTTGCTGAGAG | 47 |
Exon 9 | TTCAGCAGAGCCGCACCAG | 55 | GAACCCAACTGCCGAGGAC | 55 |
Exon 10 | CCGACCCTGTGCTCTGTGT | 55 | GTGCTGCGGTTCTGTCTGG | 55 |
Exon 11 | GGGTTGAATTTTAGGGACTC | 50 | CACAGCCACCAAACAAGAGG | 54 |
Exon 12 | ACTGATGCCACCTCTTCTCC | 54 | CTCCAGCCACAGTGACAACC | 56 |
Exon 13 | GCCTGACCTCCGTATCTGCT | 56 | ACACCCACGGAGCCTTTCTG | 56 |
Exon 14 | GTGAGGGTGCTGGTAGGTGA | 56 | CTGGGGGCTAAGAACACTGA | 54 |