Gene

Primer sequence (5′ → 3′)

TYMS

Forward: TCTGCTGACAACCAAACGTGTGTTC

Reverse: CCATTGGCATCCCAGATTTTCAC

SHMT1

Forward: CCAGAGATACTATGGCGGGACTGAG

Reverse: CCAGCACTGTGGGTCCAGCTTATAG

DHFR

Forward: AGTCAGCGAGCAGGTTCTCATTGA

Reverse: TGGACTATGTTCCGCCCACACA

DPYD

Forward: AGGAGGGTTTGTCACTGGCAGACT

Reverse: TTCTTGGCCGAAGTGGAACACAG

18S rRNA

Forward : TGCCCTATCAACTTTCGATGGTAGTC

Reverse: TTGGATGTGGTAGCCGTTTCTCA

DHMT1

Forward: CTGGACGACCCTGACCTCAAATATG

Reverse: CGCCTCATAACTCTCAAAGCCAGAC

ERCC1

Forward: GCTACCACAACCTGCACCCAGACT

Reverse: GCAGTCGGCCAGGATACACATCT