| No | Sample Code | Bottle capacity (mL) | PG (%) | VG (%) | Bottle material, Shelf life | pH | Labelled nicotine (mg/mL) | Quantified nicotine in solution (mg/mL) | Relative difference (%) | Free nicotine (mg/mL) | Quantified nicotine in aerosol (mg/mL) (13-puffs) | Nicotine released to aerosol from solution (%) |
| 1) Nicotine-containing refill solutions | ||||||||||||
| 1 | EN01 | 30 | 40 | 60 | P, ns | 5 | 6 | 0.224 | −96.3 | <0.001 | 0.036 | 16.1 |
| 2 | EN02 | 30 | 50 | 60 | P, ns | 5 | 6 | 0.775 | −87.1 | 0.001 | 0.060 | 7.7 |
| 3 | EN03 | 30 | 50 | 50 | P, ns | 4 | 6 | 0.855 | −85.8 | <0.001 | 0.045 | 5.3 |
| 4 | ES04 | 30 | 50 | 50 | P, ns | 5 | 6 | 2.588 | −56.9 | 0.002 | 0.138 | 5.3 |
| 5 | EN05 | 30 | ns | ns | P, ns | 5 | 6 | 2.140 | −64.3 | 0.002 | 0.123 | 5.7 |
| 6 | EN06 | 30 | 47 | 53 | G, ns | 6 | 7.5 | 3.462 | −53.8 | 0.033 | 0.291 | 8.4 |
| 7 | ES07 | 30 | 50 | 50 | P, ns | 6 | 6 | 3.707 | −38.2 | 0.035 | 0.527 | 14.2 |
| 8 | ES08 | 30 | 50 | 50 | P, ns | 6 | 6 | 3.740 | −37.7 | 0.035 | 0.373 | 10.0 |
| 9 | ES09 | 30 | 50 | 50 | P, 2018 | 6 | 6 | 3.754 | −37.4 | 0.036 | 0.140 | 3.7 |
| 10 | ES10 | 30 | 50 | 50 | P, ns | 6 | 6 | 3.791 | −36.8 | 0.036 | 0.311 | 8.2 |
| 11 | ES11 | 30 | 50 | 50 | P, ns | 6 | 6 | 3.797 | −36.7 | 0.036 | 0.040 | 1.1 |
| 12 | EN12 | 35 | ns | ns | P, ns | 5 | 6 | 3.969 | −33.9 | 0.004 | 0.376 | 9.5 |
| 13 | ES13 | 30 | 50 | 50 | P, ns | 4 | 6 | 4.092 | −31.8 | <0.001 | 0.309 | 7.6 |
| 14 | EN14 | 30 | 30 | 70 | g, ns | 5 | 6 | 4.264 | −28.9 | 0.004 | 0.238 | 5.6 |
| 15 | ES15 | 30 | 50 | 50 | P, ns | 5 | 6 | 4.517 | −24.7 | 0.004 | 0.497 | 11.0 |
| 16 | ES16 | 30 | 50 | 50 | G, ns | 5 | 6 | 4.525 | −24.6 | 0.004 | 0.230 | 5.1 |
| 17 | ES17 | 30 | ns | ns | P, ns | 6 | 6 | 4.560 | −24.0 | 0.043 | 0.457 | 10.0 |
| 18 | EN18 | 30 | ns | ns | P, ns | 5 | 5 | 4.091 | −18.2 | 0.004 | 0.363 | 8.9 |
| 19 | ES19 | 30 | 50 | 50 | P, ns | 6 | 6 | 5.039 | −16.0 | 0.048 | 0.463 | 9.2 |
| 20 | EN20 | 30 | ns | ns | P, ns | 6 | 6 | 5.428 | −9.5 | 0.051 | 0.640 | 11.8 |
| 21 | EN21 | 55 | Low | High | P, ns | 5 | 3 | 2.853 | −4.9 | 0.003 | 0.169 | 5.9 |
| 22 | EN22 | 30 | 60 | 40 | P, ns | 4 | 6 | 5.875 | −2.1 | 0.001 | 0.640 | 10.9 |
| 23 | EN23 | 30 | 50 | 50 | P, ns | 4 | 6 | 5.989 | −0.2 | 0.001 | 0.445 | 7.4 |
| 24 | ES24 | 30 | 40 | 60 | G, ns | 6 | 12 | 12.033 | 0.3 | 0.114 | 0.166 | 1.4 |
| 25 | ES25 | 50 | ns | ns | G, ns | 5 | 9 | 9.129 | 1.4 | 0.009 | 0.702 | 7.7 |
| 26 | ES26 | 55 | 50 | 50 | P, ns | 5 | 6 | 6.233 | 3.9 | 0.006 | 0.518 | 8.3 |
| 27 | ES27 | 30 | 60 | 40 | P, ns | 5 | 6 | 6.573 | 9.6 | 0.006 | 0.535 | 8.1 |
| 28 | ES28 | 30 | 50 | 50 | P, ns | 6 | 9 | 10.777 | 19.7 | 0.102 | 0.762 | 7.1 |
| 29 | ES29 | 30 | 60 | 40 | G, ns | 5 | 12 | 14.880 | 24.0 | 0.014 | 1.165 | 7.8 |
| 30 | ES30 | 30 | ns | ns | G, ns | 6 | 12 | 14.989 | 24.9 | 0.142 | 1.167 | 7.8 |
| 31 | ES31 | 30 | 50 | 50 | G, ns | 6 | 9 | 11.356 | 26.2 | 0.107 | 1.056 | 9.3 |
| 32 | ES32 | 30 | ns | ns | P, ns | 5 | 6 | 7.580 | 26.3 | 0.007 | 0.100 | 1.3 |
| 33 | ES33 | 30 | ns | 33 | P, ns | 6 | 12 | 15.292 | 27.4 | 0.145 | 1.563 | 10.2 |
| 34 | ES34 | 30 | ns | 33 | P, ns | 5 | 9 | 11.570 | 28.6 | 0.011 | 0.973 | 8.4 |