Analytical equipment |
|
Nicotine in refill solution | GC-2010 gas chromatograph equipped with QP2010 ultra mass spectrometer (Shimadzu Corporation) |
Nicotine in aerosol | GC-2010 gas chromatograph equipped with TQ8040 mass spectrometer (Shimadzu Corporation) |
Auto sampler/injector | AOC-20s Auto sampler and AOC-20i Auto injector |
Column | SH-Rxi-5Sil MS (30m, 0.25 mm ID, 0.25 µm) (Shimadzu Corporation) |
Injection mode | Split 20:1 |
Injection temperature | 280˚C |
Injection pressure | 200 kP for 1 minute |
Carrier gas | Helium (Purity 99.999%), constant flow |
Flow rate | 1.0 mL/min |
Linear velocity | 37.2 cm/sec |
Oven | 100˚C was held for 1 minute then ramped at rate of 30˚C/min to 280˚C, which was further held for 4 minutes |
Run time | 11 minutes |
Solvent delay | 2.5 minutes |
Quantitation mode | Selected Ion Monitoring (SIM) |
Source temperature | 200˚C |
Interface temperature | 280˚C |
Acquisition mode | m/z 50 - 500, confirmed with the NIST14 specteral library |
Target ion for nicotine in refill solution | m/z 84 (nicotine) and reerence ions m/z 133 and m/z 161 |
Target ion for nicotine in aerosol solution | m/z 84 (nicotine) and reerence ions m/z 133 and m/z 162 |
Target ion for IS | m/z 194 (caffeine) and reference ions m/z 109 and m/z 55 |
Ion tolerance in SIM mode | 30% |