Analytical equipment

Nicotine in refill solution

GC-2010 gas chromatograph equipped with QP2010 ultra mass spectrometer (Shimadzu Corporation)

Nicotine in aerosol

GC-2010 gas chromatograph equipped with TQ8040 mass spectrometer (Shimadzu Corporation)

Auto sampler/injector

AOC-20s Auto sampler and AOC-20i Auto injector

Column

SH-Rxi-5Sil MS (30m, 0.25 mm ID, 0.25 µm) (Shimadzu Corporation)

Injection mode

Split 20:1

Injection temperature

280˚C

Injection pressure

200 kP for 1 minute

Carrier gas

Helium (Purity 99.999%), constant flow

Flow rate

1.0 mL/min

Linear velocity

37.2 cm/sec

Oven

100˚C was held for 1 minute then ramped at rate of 30˚C/min to 280˚C, which was further held for 4 minutes

Run time

11 minutes

Solvent delay

2.5 minutes

Quantitation mode

Selected Ion Monitoring (SIM)

Source temperature

200˚C

Interface temperature

280˚C

Acquisition mode

m/z 50 - 500, confirmed with the NIST14 specteral library

Target ion for nicotine in refill solution

m/z 84 (nicotine) and reerence ions m/z 133 and m/z 161

Target ion for nicotine in aerosol solution

m/z 84 (nicotine) and reerence ions m/z 133 and

m/z 162

Target ion for IS

m/z 194 (caffeine) and reference ions m/z 109 and m/z 55

Ion tolerance in SIM mode

30%