Source of β-glucan

Extraction method






of Publication

Publication Year


Saccharomyces cerevisiae dry yeast cells

-The acid-base extraction method (NaOH, 80˚C, 2 h, CH3COOH)

Spectroscopicanalysis FTIR

Enhancement of β-Glucan Biological Activity Using a Modified Acid-Base Extraction Method from Saccharomyces cerevisiae.

Biotechnological β-glucan Production from Returned Baker’s Yeast and Yeast Remaining after Ethanol Fermentation

Mahmoud Amer E, Saber SH, Abo Markeb A,

Zohri AE-N, Moubasher H, Abdel-Hay H, Orban M


Egyptian Sugar Journal





Saccharomyces cerevisiae pure culture

-Modified acid-base extraction method (NaOH, 80˚C, 2 h, DMSO)

Spectroscopic analysis FTIR

Extraction of β-glucan from Saccharomyces

cerevisiae: Comparison of different extraction methods and in vivo assessment of immunomodulatory effect in mice

Pengkumsri N, Sivamaruthi BS, Sirilun S,

Food Sci Technol



Saccharomyces cerevisiae, pure culture

NRRL Y-567

-Alkaline-acid extraction method assisted with ultrasound (NaOH, 90˚C, 1 h, 40 kHz during 15 min, CH3COOH, 85˚C, 1 h,

-Ultrasound-assisted autolysis (50˚C, 24 h, 40 kHz during 15 min, CH3COOH, 85˚C, 1 h

Spectroscopic analysis FTIR

A new isolation method of β-d-glucans from spent yeast Saccharomyces cerevisiae

Liu X, Wang Q, Cui S, Liu H

Food Hydrocolloids



Saccharomyces cerevisiae residual yeasts from beer production

-Induced autolysis (3% NaCl, 55˚C, 24 h) + treatment with water and organic solvent (121˚C, 4 h), homogenization and protease hydrolysis

Electron microscopy

Optimized methodology for extraction of (1 → 3)(1 → 6)-β-d-glucan from Saccharomyces cerevisiae and in vitro evaluation of the cytotoxicity and genotoxicity of the corresponding carboxymethyl derivative

Application of different methods for the extraction of yeast β-glucan

Magnani M, Calliari CM, de Macedo FC

VassileiosVarelas, P. Tataridis, M. Liouni, T. Nerantzis

Carbohydrate Polymers

e-Journal of Science & Technology





Saccharomyces cerevisiae VIN 13 S

-Enzyme treatment + NaOH

-Induced autolysis (3% NaCl), hot water extraction, sonication, extraction of lipids and proteins with the help of enzymes

Enzymatic assay kit (Megazyme International)

Effect of preparation methods on physiochemical and functional properties of yeast β-glucan

Fu W, Zhao G, Liu J




Saccharomyces cerevisiae dry yeast cells Angel Yeast Co., Ltd (China)

-Autolysis (55˚C, 48 h), hot water extraction (121˚C, 6 h), deproteinization treatment (proteases)

Enzymatic assay kit (Megazyme International)

Enzymatic process for the fractionation of baker’s yeast cell wall (Saccharomyces cerevisiae)

Borchani C, Fonteyn F, Jamin G

Food Chemistry



Saccharomyces cerevisiae cell wall in the form of powder

-Extraction by the enzymatic method (hot water extraction 125˚C, 5 h, treatment with proteases, treatment with lipases)

Enzymatic assay kit (Megazyme International)

New method for preparing purity β-D-glucans (beta-Glucan) from baker’s yeast (Saccharomyces cerevisiae)

Khanh Pham, Nguyen Nhut, Nguyen Cuong




Saccharomyces cerevisiae baker’s yeast powder Saf-Viet

-Extraction using the ionic solution (ionic solution 1-butyl-3-methyl- imidazolium Chloride, stirring 30 min at 80˚C)

1D- and

2D-NMR spectroscopy

Optimization of β-glucan extraction from waste brewer’s yeast saccharomyces cerevisiae using autolysis, enzyme, ultrasonic and combined enzyme – ultrasonic treatment

Tran Minh Tam, Nguyen Quoc Duy

American Journal of Research Communication



Saccharomyces cerevisiae residual yeasts from beer production

-Combined enzymatic and ultrasound extraction

Enzymatic assay kit (Megazyme International)

A Simple and Efficient Mechanical Cell Disruption Method Using Glass Beads to Extract β-Glucans from Spent Brewer’s Yeast

Avramia I, Amariei S

Applied Sciences



Saccharomyces cerevisiae residual yeasts from beer production

-Mechanical damage of yeast cells, acid-base extraction

Spectroscopicanalysis FTIR

Antioxidant Activity of β-Glucan

Kofuji K, Aoki A, Tsubaki K

ISRN Pharmaceutics