Source of β-glucan	Extraction method	Analysismethod	Literature
Source of β-glucan	Extraction method	Analysismethod	Title	Authors	Journal of Publication	Publication Year	References
Saccharomyces cerevisiae dry yeast cells	-The acid-base extraction method (NaOH, 80˚C, 2 h, CH₃COOH)	Spectroscopicanalysis FTIR	Enhancement of β-Glucan Biological Activity Using a Modified Acid-Base Extraction Method from Saccharomyces cerevisiae. Biotechnological β-glucan Production from Returned Baker’s Yeast and Yeast Remaining after Ethanol Fermentation	Mahmoud Amer E, Saber SH, Abo Markeb A, Zohri AE-N, Moubasher H, Abdel-Hay H, Orban M	Molecules Egyptian Sugar Journal	2021 2019	[62] [63]
Saccharomyces cerevisiae pure culture	-Modified acid-base extraction method (NaOH, 80˚C, 2 h, DMSO)	Spectroscopic analysis FTIR	Extraction of β-glucan from Saccharomyces cerevisiae: Comparison of different extraction methods and in vivo assessment of immunomodulatory effect in mice	Pengkumsri N, Sivamaruthi BS, Sirilun S,	Food Sci Technol	2016	[64]
Saccharomyces cerevisiae, pure culture NRRL Y-567	-Alkaline-acid extraction method assisted with ultrasound (NaOH, 90˚C, 1 h, 40 kHz during 15 min, CH₃COOH, 85˚C, 1 h, -Ultrasound-assisted autolysis (50˚C, 24 h, 40 kHz during 15 min, CH₃COOH, 85˚C, 1 h	Spectroscopic analysis FTIR	A new isolation method of β-d-glucans from spent yeast Saccharomyces cerevisiae	Liu X, Wang Q, Cui S, Liu H	Food Hydrocolloids	2008	[65]
Saccharomyces cerevisiae residual yeasts from beer production	-Induced autolysis (3% NaCl, 55˚C, 24 h) + treatment with water and organic solvent (121˚C, 4 h), homogenization and protease hydrolysis	Electron microscopy	Optimized methodology for extraction of (1 → 3)(1 → 6)-β-d-glucan from Saccharomyces cerevisiae and in vitro evaluation of the cytotoxicity and genotoxicity of the corresponding carboxymethyl derivative Application of different methods for the extraction of yeast β-glucan	Magnani M, Calliari CM, de Macedo FC VassileiosVarelas, P. Tataridis, M. Liouni, T. Nerantzis	Carbohydrate Polymers e-Journal of Science & Technology	2009 2016	[66] [67]
Saccharomyces cerevisiae VIN 13 S	-Enzyme treatment + NaOH -Induced autolysis (3% NaCl), hot water extraction, sonication, extraction of lipids and proteins with the help of enzymes	Enzymatic assay kit (Megazyme International)	Effect of preparation methods on physiochemical and functional properties of yeast β-glucan	Fu W, Zhao G, Liu J	LWT	2022	[56]
Saccharomyces cerevisiae dry yeast cells Angel Yeast Co., Ltd (China)	-Autolysis (55˚C, 48 h), hot water extraction (121˚C, 6 h), deproteinization treatment (proteases)	Enzymatic assay kit (Megazyme International)	Enzymatic process for the fractionation of baker’s yeast cell wall (Saccharomyces cerevisiae)	Borchani C, Fonteyn F, Jamin G	Food Chemistry	2014	[68]
Saccharomyces cerevisiae cell wall in the form of powder	-Extraction by the enzymatic method (hot water extraction 125˚C, 5 h, treatment with proteases, treatment with lipases)	Enzymatic assay kit (Megazyme International)	New method for preparing purity β-D-glucans (beta-Glucan) from baker’s yeast (Saccharomyces cerevisiae)	Khanh Pham, Nguyen Nhut, Nguyen Cuong	STDJ	2020	[69]
Saccharomyces cerevisiae baker’s yeast powder Saf-Viet	-Extraction using the ionic solution (ionic solution 1-butyl-3-methyl- imidazolium Chloride, stirring 30 min at 80˚C)	1D- and 2D-NMR spectroscopy	Optimization of β-glucan extraction from waste brewer’s yeast saccharomyces cerevisiae using autolysis, enzyme, ultrasonic and combined enzyme – ultrasonic treatment	Tran Minh Tam, Nguyen Quoc Duy	American Journal of Research Communication	2013	[70]
Saccharomyces cerevisiae residual yeasts from beer production	-Combined enzymatic and ultrasound extraction	Enzymatic assay kit (Megazyme International)	A Simple and Efficient Mechanical Cell Disruption Method Using Glass Beads to Extract β-Glucans from Spent Brewer’s Yeast	Avramia I, Amariei S	Applied Sciences	2022	[71]
Saccharomyces cerevisiae residual yeasts from beer production	-Mechanical damage of yeast cells, acid-base extraction	Spectroscopicanalysis FTIR	Antioxidant Activity of β-Glucan	Kofuji K, Aoki A, Tsubaki K	ISRN Pharmaceutics	2012	[72]