| Time (min) | Solvent Α (% v/v) | Solvent Β (% v/v) | Flow (ml/min) |
| 0 | 100 | 0 | 1 |
| 30 | 85 | 15 | 1 |
| 40 | 78 | 22 | 1 |
| 50 | 0 | 100 | 1 |
| 65 | 0 | 100 | 1 |
| 70 | 100 | 0 | 1 |