| Time (min) | Composition of mobile phase (%) | |
| Water | Acetonitrile | |
| 0 | 80 | 20 |
| 5 | 80 | 20 |
| 25 | 72 | 28 |
| 35 | 67 | 33 |
| 40 | 59 | 41 |
| 45 | 20 | 80 |
| 47 | 20 | 80 |
| 50 | 80 | 20 |