Time (min) | Composition of mobile phase (%) | |
Water | Acetonitrile | |
0 | 80 | 20 |
5 | 80 | 20 |
25 | 72 | 28 |
35 | 67 | 33 |
40 | 59 | 41 |
45 | 20 | 80 |
47 | 20 | 80 |
50 | 80 | 20 |