Matrix

Concentration (µg/ml)

Concentration predicted^a (µg/ml)

Mean prediction error (%)

CV%

Perfusate^b

1

1.17 ± 0.10

17.50

9.31

2

2.18 ± 0.22

9.23

10.00

3

3.04 ± 0.09

1.37

3.03

5

4.56 ± 0.29

−8.77

6.50

10

9.64 ± 0.66

−3.57

6.84

20

20.6 ± 1.00

2.94

4.88

40

40.6 ± 0.94

1.43

2.32

KHS buffer

10

10.6 ± 1.15

6.59

10.80

15

15.5 ± 0.35

3.45

2.27

20

20.8 ± 1.04

3.74

4.99

40

39.2 ± 1.35

−2.04

3.45

60

59.3 ± 1.88

−1.12

3.17

80

79.3 ± 2.09

−0.82

2.63

100

103 ± 2.74

3.15

2.65