Group

First time

6 months

12 months

24 months

Wheal

Erythema

Wheal

(p value)

Erythema

(p value)

Wheal

(p value)

Erythema

(p value)

Wheal

(p value)

Erythema

(p value)

N1

0.84 ± 0.24

2.01 ± 0.86

0.64 ± 0.28

(0.024)

1.33 ± 0.66

(0.001)*

0.72 ± 0.22

(0.111)

1.42 ± 0.63

(0.001)*

0.77 ± 0.13

(0.83)

1.56 ± 0.48

(0.008)*

N2

0.96 ± 0.35

2.70 ± 0.95

0.76 ± 0.28

(0.002)*

2.25 ± 0.76

(0.006)*

0.92 ± 0.22

(0.111)

2.26 ± 0.74

(0.005)*

0.92 ± 0.29

(0.233)

2.37 ± 0.70

(0.029)

N3

0.97 ± 0.35

2.42 ± 0.83

0.84 ± 0.30 (0.008)*

1.54 ± 0.64

(0.001)*

0.88 ± 0.33

(0.019)

2.00 ± 0.70

(0.026)

0.90 ± 0.34

(0.203)

1.51 ± 0.57

(0.001)*

N4

1.06 ± 0.42

2.46 ± 1.37

0.98 ± 0.36

(0.072)

1.55 ± 0.64

(0.173)

1.00 ± 0.44

(0.634)

1.95 ± 1.34

(0.415)

0.88 ± 0.41

(0.024)

1.75 ± 0.90

(0.031)