| Strain/plasmid/ oligonucleotide | Phenotype/Genotype/Description | Source or reference |
| Bacterial strains |
|
|
| R. sphaeroides 2. 4. 1 | Wild type | Van Niel (1944) |
| R. sphaeroides ΔctrA | 2. 4. 1 derivative with in-frame deletion of the DNA sequence between codon 7 and 231 from the start codon of ctrA | This study |
| E. coli DH5α-phe | (Ф80dlacZ¢M15) ¢lacU169 recA1 endA1 hsdR17 supE44 11 thi1 gyrA96 relA1 phe::Tn10dCm | (10) |
| E. coli S17-1 | Pro− Res− Mod+ recA; integrated plasmid RP4-Tc::Mu Km::Tn7 | Simon et al. (1983) |
| SHSU0001 | DH5α-phe containing pLO1ΔctrA plasmid | This study |
| SHSU0002 | E. coli S17-1 containing pLO1ΔctrA plasmid | This study |
| Plasmids |
|
|
| pLO1 | Kmr; sacBRP4-oriT ColE1-oriV | Lenz et al. |
| pLO1ΔctrA | pLO1::0.5 fragment of ΔctrA | This study |
| pRK415 | Tcr; Mob+lacZ IncP | Keen et al. (1988) |
| pRK415ctrA | pRK415::0.8 Kb fragment ctrA gene of R. sphaeroides | This study |
| Oligonucleotides |
|
|
| ctrA-upstream F1 | 5’-GCTCTAGAGCCGCGGAAGGTGTTCTCGTAA-3’ | This study |
| ctrA-upstream R2 | 5’-TCAGGCGCCGATGGCGAACCGCTCCACCAGCAGTATTCTCAT-3’ | This study |
| ctrA-downstream F3 | 5’-ATGAGAATACTGCTGGTGGAGCGGTTCGCCATCGGCGCCTGA-3’ | This study |
| ctrA-downstream R4 | 5’-GCTCTAGAGCGCGCCCCAGGCATAGGCGAA-3’ | This study |
| ctrA-sequencing F1 | 5’-AATTCGCCCTGTGGCGGTCG-3’ | This study |
| ctrA-sequencing R2 | 5’-TCTGCCAGCCGTGCAAGCTC-3’ | This study |
| ctrA-qPCR-1F1 | 5’-GAATACTGCTGGTGGAGGATG-3’ | This study |
| ctrA-qPCR-1R2 | 5’-ATCGTAATCGTAGAGCTTGGC-3’ | This study |
| ctrA-qPCR-2F1 | 5’-ACGATTACATGACCAAGCCC-3’ | This study |
| ctrA-qPCR-2R2 | 5’-TTCGCATCGAGATTAACGAGG-3’ | This study |
| ctrA-qPCR-3F1 | 5’-ATCACCTTTACGGAGGCATG-3’ | This study |
| ctrA-qPCR-3R2 | 5’-ACCGTCTCGATGTAGCTCTC-3’ | This study |
| rpoZ-qPCR F1 | 5’-ATCGCGGAAGAGACCCAGAG-3’ | This study |
| rpoZ-qPCR R2 | 5’-GAGCAGCGCCATCTGATCCT-3’ | This study |
| ctrA-2F | 5’-AAGCTTTTAACCATTGGGCATCATTG-3 | This study |
| ctrA-2R | 5’-TCTAGAGGCAATCCGCCCGAG-3 | This study |