| Composition | Volume (µl) | Concentration |
| ddH2O | 6 | - |
| 2x PCR Master Mix (i-Taq™) | 15 | - |
| Primer 1 (27f) | 3 | 10 pmol/µl |
| Primer 2 (1495r) | 3 | 10 pmol/µl |
| DNA template | 3 | 1 µg |