Equation

r2

Linear range (ppb)

LOD (ppb)

LOD (nM)

GLU

y = 0.0004x + 0.3042

0.9991

0.8 - 16

0.8

5.4

ALA

y = 0.0001x āˆ’ 0.0827

0.9973

1.9 - 19

1.5

16.8

MET

y = 0.0003x + 0.3377

0.9928

0.8 - 20

0.53

3.6

TRP

y = 0.0004x + 1.1147

0.9967

0.44 - 17.6

0.44

2.2

PRO

y = 0.0007x + 0.1392

0.9988

0.84 - 21

0.84

7.3

GLY

y = 3.00Eāˆ’06x + 0.0890

0.9982

19.5 - 975

19.5

260

LYS

y = 6.00Eāˆ’05x + 0.3276

0.9996

2.1 - 21

2.1

14.3

TYR

y = 0.0003x + 0.4718

0.9966

1.95 - 15.6

0.39

2.2

PHE

y = 0.0013x + 3.6841

0.9957

0.42 - 16.8

0.14

0.8

LEU

y = 0.0017x + 5.1268

0.9948

0.38 - 15.2

25 ppt

0.2