| Target gene | Primer pair | Primer sequence (5’-3’) | Fragment size (pb) | Annealing at Temperature ˚C |
| Resistance gene |
| |||
| MecA | mecA1 | AAAATCGATGGTAAAGGTTGGC | 532 | 55 |
| mecA2 | AGTTCTGCAGTACCGGATTTGC | |||
| Tetk | tetk1 | GTAGCGACAATAGGTAATAGT | 360 | |
| tetk2 | GTAGTGACAATAAACCTCCTA | |||
| TetM | tetM1 | AGTGGAGCGATTACAGAA | 158 | |
| tetM2 | CATATGTCCTGGCGTGTCTA | |||
| aacA-aphD | aacA-aphD1 | TAATCCAAGAGCAATAAGGGC | 227 | |
| aacA-aphD2 | GCCACACTATCATAACCACTA | |||
| Virulence gene |
| |||
| LukS-PV | LukS-PV | GGCCTTTCCAATACAATATTGG | 433 | 55 |
| LukF-PV | CCCAATCAACTTCATAAATTG | |||
| Tst | tst-1F | GAAATTTTTCATCGTAAGCCCTTTGTTG | 180 | |
| tst-1R | TTCATCAATATTTATAGGTGGTTTTTCA | |||