Gene and Accession Number

Primer

Amplicon Size

pKiss1 (NM_001134964.1)^a

Forward

Reverse

5'-GGCAGCTGATGTTCTTTCTTTG-3'

5'-CGGGCCTGTAGATCTAGGATT-3'

89 bp

pLH-b (NM_214080.1)^b

Forward

Reverse

5'-ATGCTCCAGAGACTGCTGTTGT-3'

5'-TGCTGGTGGTAAAGGTGATGCAGA-3'

151 bp

GnRHR (NM_214273.1)^c

Forward

Reverse

5'-AGCCAACCTGTTGGAGACTCTGAT-3'

5'-AGCTGAGGACTTTGCAGAGGAACT-3'

101 bp

pGAPDH (AF017079.1)^d

Forward

Reverse

5'-GCAAAGTGGACATTGTCGCCATCA-3'

5'-TGACTGTGCCGTGGAATTTGCCAT-3'

105 bp

pb-actin (U07786.1)^e

Forward

Reverse

5'-TCGCCGACAGGATGCAGAAGGA-3'

5'-AGGTGGACAGCGAGGCCAGGAT-3'

129 bp