Spots on TLC plate

Rf values

1

Rf1 = 0.79

Rf2 = 0.89

2

Rf1 = 0.26

Rf2 = 0.42

Rf3 = 0.58

Rf4 = 0.66

Rf5 = 0.77

Rf6 = 0.91

3

Rf1 = 0.77

Rf2 = 0.91

4

Rf1 = 0.40

Rf2 = 0.77

Rf3 = 0.91

5

Rf1 = 0.17

Rf2 = 0.32

6

Rf1 = 0