Cell capture structures

Targeted cells

Carrier medium and control cells

Target cell recovery rate

Capture purity

A nickel micropillar array + magnetic

beads functionalized with wheat germ agglutinin

Human lung cancer

cells of A549

Human RBCs + culture medium

62% - 74%

93% (initial ratio

A549:RBCs 1/4 1:10)

A paramagnetic array of 80% Ni and 20% Fe+ magnetic beads complementary to anti-CD10 antibodies in chamber 1 and anti-PSMA antibodies in chamber 2

Human prostate cancer cells of LNCaP incubated with PSMA

antibodies

LNCaP incubated with CD10 antibodies + PBS

50% - 70% of LNCaP incubated with PSMA antibodies in chamber 2

10% of LNCaP

incubated with CD10

antibodies in chamber 2

(initial mixture ratio of

1:1)

External permanent magnet + magnetic beads coated with Anti-EpCAM (Ber-EP4)

Human ovarian cancer cells of BG-1 and lung cancer cells of AS2

Blood samples from healthy donors (106 cells per ml)

95.1% for BG-1 cells and 92.7% for AS2 cells

NA

An array of magnetic dots + self-assembled magnetic beads coated with anti-CD19 antibodies

Human lymphoma

cells of Raji CCL-86

Human lymphoma cells of Jurkat TIB152 + PBS (2 × 106 cells per ml)

97% ± 2% of Raji cells

<2% (capture of Jurkat TIB152 cells)

An array of magnetic dots + self-assembled magnetic beads coated with

anti-CD19 antibodies

B-cell hematological

malignant tumors

(leukemia and

lymphoma)

Clinical samples (blood, pleural

effusion, and fine needle aspirates) from chronic lymphocytic

leukemia, mantle cell lymphoma,

follicular lymphoma and two

healthy volunteers

Consistent immunophenotype and morphology results with those obtained by flow cytometry

External permanent magnet + self-assembled magnetic bead patterns

coated with 5D10 antibodies

Human breast cancer

cells of MCF-7

Human lymphoma cells of Jurkat TIB152 + PBS (106cells per ml for both MCF-7 and Jurkat cells)

85% ± 10% of MCF-7

<5% (capture of Jurkat

TIB152 cells)

A nickel micropillar array + magnetic

beads functionalized with wheat germ

agglutinin

Human lung cancer

cells of A549

PBS

A total mass of 90.6 ng of captured A549 cells

External permanent magnet + Fe3O4

magnetic nanoparticles conjugated to

Anti-EpCAM

Human colon cancer

cells of COLO205 and

human breast cancer

cells of SK-BR-3

Blood samples from healthy donors

90% and 86% for

COLO205 and SK-BR-3

cells, respectively

NA