Gene

Primer Sequence (5’ → 3’ )

TYMS

Forward: TCTGCTGACAACCAAACGTGTGTTC

Reverse: CCATTGGCATCCCAGATTTTCAC

SHMT1

Forward: CCAGAGATACTATGGCGGGACTGAG

Reverse: CCAGCACTGTGGGTCCAGCTTATAG

DHFR

Forward: AGTCAGCGAGCAGGTTCTCATTGA

Reverse: TGGACTATGTTCCGCCCACACA

DPYD

Forward: AGGAGGGTTTGTCACTGGCAGACT

Reverse: TTCTTGGCCGAAGTGGAACACAG

18S rRNA

Forward : TGCCCTATCAACTTTCGATGGTAGTC

Reverse: TTGGATGTGGTAGCCGTTTCTCA

NANOG

Forward: TGAGATGCCTCACACGGAGACTG

Reverse: GGGTTGTTTGCCTTTGGGACTG

DHMT1

Forward: CTGGACGACCCTGACCTCAAATATG

Reverse: CGCCTCATAACTCTCAAAGCCAGAC

ERCC1

Forward: GCTACCACAACCTGCACCCAGACT

Reverse: GCAGTCGGCCAGGATACACATCT