| Sequence |
1st PCR: |
|
SD + GFP (F)1) | 5-’cgacTAGGAGGTTTAAATTTATGGTttctAAGGGCGAGGAGCTGTTCACC-3’ |
GFP + Term + TSR (R)2) | 5’-gtgctcgaGATATCATCTAGAGGATCCGACTAGTGATaagCTTACTTGTACAGCTCGTCC3-’ |
2nd PCR: |
|
NcoI + TSL + SD + Met (F)3) | 5’-aggagatataccatgGATATCACTAGTCGGATCgtcgacTAGGAGGTTTAAATTTATGG-3’ |
TSR + XhoI (R) | 5’-ggtggtggtgctcgaGATATCATCTAGAGG-3’ |