| Chemicals | Concentration | Activity (%) |
| None | - | 100 |
| PMSF | 5 mM | 80 ± 1.36 |
| EDTA | 5 mM | 0 |
| β-mercaptoethanol | 5 mM | 92 ± 1 |
| DNTB | 5 mM | 100 |
| Ca2+ | 5 mM | 84 ± 2 |
| Ba2+ | 5 mM | 54 ± 1 |
| Zn2+ | 5 mM | 89.5 ± 1.2 |
| Cu2+ | 5 mM | 48.62 ± 1.62 |
| Mg2+ | 5 mM | 84 ± 2.2 |
| Mn2+ | 5 mM | 78.54 ± 2.4 |
| K+ | 5 mM | 87.8 ± 2.1 |
| Na+ | 5 mM | 85 ± 1.8 |
| Hg2+ | 5 mM | 0 |
| Fe2+ | 5 mM | 27 ± 2 |
| Cu2+ |
| 71 ± 1.5 |