Measured parameter

Previous process1

Optimized process2

Limonene (96% purity)

Limonene (97% purity)

Orange oil (89% limonene)

Bioconversion conditions

25˚C; pH 7.1; 10 g∙L cell mass (dry weight)

25˚C; pH 6.9; 20 g/L cell mass (dry weight)

Total limonene amount (mM)3

29.7 (4.0 g∙L−1)

19.2 (2.6 g∙L−1)

17.6 (2.4 g∙L−1)

Limonene addition mode over 48 h

6 additions

2 additions

2 additions

Final PA concentration

0.855 g∙L−1

0.796 g∙L−1

0.872 g.L−1

PA molar yield

16.7%

25,0%

29.7%

Cost of limonene substrate to produce 1 Kg PA

552.38 USD4

386.67 USD4

80.56 USD4

12.90 USD5