Measured parameter | Previous process1 | Optimized process2 | |
Limonene (96% purity) | Limonene (97% purity) | Orange oil (89% limonene) | |
Bioconversion conditions | 25˚C; pH 7.1; 10 g∙L cell mass (dry weight) | 25˚C; pH 6.9; 20 g/L cell mass (dry weight) | |
Total limonene amount (mM)3 | 29.7 (4.0 g∙L−1) | 19.2 (2.6 g∙L−1) | 17.6 (2.4 g∙L−1) |
Limonene addition mode over 48 h | 6 additions | 2 additions | 2 additions |
Final PA concentration | 0.855 g∙L−1 | 0.796 g∙L−1 | 0.872 g.L−1 |
PA molar yield | 16.7% | 25,0% | 29.7% |
Cost of limonene substrate to produce 1 Kg PA | 552.38 USD4 | 386.67 USD4 | 80.56 USD4 12.90 USD5 |