Chinese medicines | Alkaloids | Adsorbent | Elution Process | Purity | Recovery | Reference | ||
Type | Mesh/Particle Size | Eluent Composition | Elution Method | |||||
Lindera aggregata | several fractions of aconite alkaloids | C18-reversed silica gel |
| methanol - water | gradient elution |
|
| [56] |
Clausena anisum-olens | 8 fractions of aconite alkaloids | silica gel |
| petroleum ether - ethyl acetate ethyl acetate ethyl acetate - methanol methanol | gradient elution |
|
| [61] |
Angelica dahurica | 20 fractions of aconite alkaloids | RP-C18-reversed silica gel |
| methanol - water | gradient elution |
|
| [58] |
Rauvolfia yunnanensis | 2 fractions of aconite alkaloids | silica gel |
| chloroform - methanol | gradient elution |
|
| [54] |
Herba Aconiti | heteratisine, hordenine, talatisamine | silica gel |
| cyclohexane - acetone | gradient elution | all >98% |
| [58] |
Semen holarrhenae | total alkaloids (containing three alkaloid monomers) | silica gel | 200 - 300 mesh | chloroform - methanol | gradient elution | chemical compound 1: 89.23%, chemical compound 2: 94.89%, chemical compound 3: 62.64% |
| [55] |
Fritillary | 4 fractions of aconite alkaloids | silica gel | 200 - 300 mesh | petroleum ether - acetone | gradient elution |
|
| [62] |
suchengbeisine | silica gel | 200 - 300 mesh | chloroform - methanol | gradient elution |
|
| ||
verticinone-N-oxide | silica gel | 200 - 300 mesh | chloroform - methanol | gradient elution |
|
| ||
Corydalis saxicola | total alkaloids | silica gel | 200 - 300 mesh | chloroform - methanol | gradient elution |
|
| [63] |
Aconitum taipeicum | total alkaloids | silica gel |
| chloroform - methanol | gradient elution |
|
| [64] |
Semen plantaginis | 7 fractions of aconite alkaloids | silica gel |
| chloroform - methanol - ammonium hydroxide | isocratic elution |
|
| [59] |
Catharanthus roseus | vincristine, vincaleukoblastinum | silica gel |
| carrene - methanol | gradient elution | vincristine: 97.26%, vincaleukoblastinum: 94.18% | vincristine: 61.12%, vincaleukoblastinum: 58.50% | [65] |
Sophora alopecuroide | N-oxysophocarpine, oxymatrine | silica gel | 200 - 300 mesh | chloroform – methanol - ammonium hydroxide | isocratic elution | >99% |
| [60] |
sophoridine | silica gel | 200 - 300 mesh | Acetone - methanol | isocratic elution | 95.80% |
| ||
Sophora alopecuroide | matrine, oxymatrine | silica gel | 300 - 400 mesh | chloroform, chloroform - methanol | isocratic elution | matrine: >95%, oxymatrine: >95% | matrine: 22.2%, oxymatrine: 42.6% | [66] |