Factor	Parity of dam (number of samples)
	1 (90)		2 (94)		3 (89)		3 + (92)		P-value
	LSM¹	SEM²	LSM	SEM	LSM	SEM	LSM	SEM	P-value
Volume, L	5.9		7		7.4		7.1
IgG, mg/ml	36.9	1.48	35.6	1.39	36.3	1.41	37.53	2.31	0.08^NS3
IgM, mg/ml	5.6	0.36	5.7	0.34	6.7	0.35	7.2	0.56	0.14^NS
Fat, %	6.7^a	0.36	4.03^b	0.32	4.1^b	0.34	3.87^b	0.31	<0.001
Protein, %	18.5^b	0.53	17.2^b	0.49	18.48^b	0.51	19.25^a	0.47	0.04
Lactose, %	2.0^ab	0.1	2.2^a	0.09	1.9^b	0.09	1.84^b	0.08	0.02
Total solid, %	29.2^a	0.6	25.48^c	0.55	26.4^c	0.57	27.16^b	0.53	0.003
Ca, mg/kg	3015^a	95	2493^b	86	2523^b	90	2610^b	83	0.001
P, mg/kg	2146^a	94	1982^b	86	1921^b	89	1931^b	83	0.07