| Compound | Concentration spiked (ng/mL) | Room temperature for 6 h | −80˚C for 10 days | Three times freeze-thaw cylcles | Processed samples in atuosampler for 8 h | ||||
| RE % | RSD % | RE % | RSD % | RE % | RSD % | RE % | RSD % | ||
| catalpol | 40 | 3.6 | 4.1 | −1.9 | 6.3 | 4.1 | 9.4 | 1.4 | 3.9 |
| 400 | 2.7 | 3.9 | −5.1 | 5.4 | −0.9 | 11.2 | −3.3 | 4.2 | |
| 4000 | 3.2 | 7.1 | −3.8 | 5.9 | 4.9 | 3.3 | −4.1 | 13.1 | |
| harpagide | 20 | 1.1 | 5.9 | 0.3 | 7.0 | 6.7 | 12.1 | −0.9 | 2.2 |
| 200 | 4.8 | 3.1 | −4.1 | 3.9 | 2.0 | 7.8 | −3.2 | 4.9 | |
| 2000 | −2.7 | 4.7 | −6.3 | 9.7 | 4.7 | 2.9 | −5.9 | 7.1 | |