| compound | Concentration spiked (ng/mL) | Intra-day | Inter-day | Accuracy | Recovery | Matrix effect |
| RSD % | RSD % | (RE %) | (%, mean ± SD) | (%, mean ± SD) | ||
| catalpol | 40 | 7.9 | 12.9 | 6.5 | 81.2 ± 10.4 | 94.3 ± 6.9 |
| 400 | 5 | 6.6 | 4.2 | 79.4 ± 4.3 | 103.6 ± 3.7 | |
| 4000 | 3.3 | 6.8 | -5.9 | 80.6 ± 6.1 | 99.8 ± 4.8 | |
| harpagide | 20 | 8.8 | 5.2 | 2.2 | 83.1 ± 5.7 | 97.1 ± 9.7 |
| 200 | 4.5 | 5.2 | 5.5 | 80.8 ± 2.9 | 108.2 ± 3.4 | |
| 2000 | 4.8 | 13.2 | 3.9 | 78.9 ± 3.5 | 105.5 ± 6.5 | |
| IS | 200 | - | - | - | 82.7 ± 5.4 | 100.9 ± 4.7 |