| Source | Sum of squares | Degrees of freedom | Middle square | F-ratio | P-value |
| A: Fermentation time | 8.30281 | 1 | 8.30281 | 11.81 | 0.0026 |
| B: pH | 79.1911 | 1 | 79.1911 | 112.67 | 0 |
| C: Concentration | 40.2753 | 1 | 40.2753 | 57.3 | 0 |
| D: Time of hydrolysis | 99.7578 | 1 | 99.7578 | 141.93 | 0 |
| AB | 0.945313 | 1 | 0.945313 | 1.34 | 0.2596 |
| AC | 1.55761 | 1 | 1.55761 | 2.22 | 0.1522 |
| AD | 2.07061 | 1 | 2.07061 | 2.95 | 0.1015 |
| BC | 0.227813 | 1 | 0.227813 | 0.32 | 0.5755 |
| BD | 48.2653 | 1 | 48.2653 | 68.67 | 0 |
| CD | 0.73813 | 1 | 0.73813 | 1.05 | 0.3177 |
| Blocks | 0.01362125 | 1 | 0.01362125 | 0.02 | 0.8907 |
| Total error | 14.0568 | 20 | 0.702842 | ||
| Total | 295.402 | 31 | |||
| Description | Value | ||||
| R-Square | 95.2412 | ||||
| Standard error | 0.838357 | ||||
| Absolute error | 0.492891 |