| Experimental runs | pH | t (h) | x1 | x2 | MCA (U/mL) |
| 1 | 2.5 | 24 | −1 | −1 | 3.28 |
| 2 | 2.5 | 72 | −1 | 1 | 14.77 |
| 3 | 4.3 | 24 | 1 | −1 | 685.71 |
| 4 | 4.3 | 72 | 1 | 1 | 296.30 |
| 5 | 3.4 | 48 | 0 | 0 | 266.67 |
| 6 | 2.12 | 48 | −1.414 | 0 | 15.96 |
| 7 | 4.6 | 48 | 1.414 | 0 | 400.00 |
| 8 | 3.4 | 14 | 0 | −1.414 | 352.94 |
| 9 | 3.4 | 82 | 0 | 1.414 | 203.39 |
| 10 | 3.4 | 48 | 0 | 0 | 252.63 |