Primers | Forward Sequence (5’-3’) (FDXRNAi) | Reverse Sequence (5’-3’) (RDXRNAi) | Annealing Temperature |
Sense strand | GCCTCGAGAAGTTATTGAAGCTCACTATCT(Xho1) | ACGAATTCGCATAGAGGGTACTTCACATT (EcoRI) | 55˚C for 30 seconds |
Anti-sense strand | ACGGATCCAAGTTATTGAAGCTCACTATCT (BamH1) | TTAAGCTTGCATAGAAGGGTACTTCACATT (HindIII) | 55˚C for 30 seconds |