Condition

Optimum parameter

Plant age

4 weeks after subculture

Enzyme treatment

2% Cellulase + 1.5% Macerozyme

Incubation time

6 h

Centrifugation speed

1000 rpm for 10 min

Shaker (rotator) speed

55 rpm

Osmoticum (mannitol) concentration

0.7 M

Temprature

Room Temprature (25˚C ± 2˚C)

pH

5.8