| Gene | Primer name | Primer sequences (5’-3’) | Length (bp) |
|
| 408 | 408-F | CTGTACTCCAAGATGCCTACC | 131 | Real-time PCR assay |
| 408-R | CGTGCCTATCATGCTTGTTTC | |||
| PAP | PAP-F | AGCCAGTAATCGGAGTCAATG | 119 | |
| PAP-R | TCATCTTTCAATAACCCCGCC | |||
| MSP | MSP-F | AGACATGTGCCATTTTAAGTGC | 96 | |
| MSP-R | TCTATCTGTTATGCGAATCGTGT | |||
| 377 | 377-F | CCAAGAGAACTGTAGAAAGGCG | 147 | |
| 377-R | AGAAGTATAACCTCCCCACTCG | |||
| 24A | 24A-F | GGGTGGAGGGGATGTAAAATT | 113 | |
| 24A-R | GACAGATAATATTCCGCCTAAAATAGC | |||
| fATP | fATP-F | ATAGCGGATTCTGTTCGTAGC | 136 | |
| fATP-R | ATCAGCACTCCAAGCCTTATC | |||
| 18S rRNA | 18S rRNA-F | TCGGGTGTTTTCACGTCTCA | 120 | |
| 18S rRNA-R | TGGATGCCGCTGGGAAGC | |||
| 408 | 408-gF | CGCGTCGACTTGCATCGTAAGCGCC (Sal I) | 423 | Recombinant plasmids construction |
| 408-gR | CGCACTAGTCCTGACGGGAGGAGAGGAG (Spe I) | |||
| pap | pap-gF | CGCGTCGACATGAGGTATTTGCAACGCAC (Sal I) | 1440 | |
| pap-gR | CGCGGATCCTTTATAAATAAAACCAATTGCACC (BamH I) | |||
| GV1300 | 1300-F | AACTTGTGGCCGTTTACGTCG | 207 | Primers for GV1300 |
| 1300-R | TTTGGAGAGAACACGGGGGAC |