| Boundary | Catchment basin | Area (acres) | Runoff coefficient |
| Withlacoochee River at Pinetta, GA | Quitman | 376,590 | 0.75 |
| Valdosta | 180,540 | 0.75 | |
| Alapaha River at Statenville, GA | Ashburn | 327,440 | 0.60 |
| Tifton | 352,730 | 0.60 | |
| Valdosta | 362,130 | 0.60 | |
| Suwannee River at White Springs, FL | Jasper | 564,960 | 0.75 |
| Lake City | 183,750 | 0.75 | |
| Folkston | 693,610 | 0.75 | |
| Santa Fe River at High Springs, FL | High Springs | 429,410 | 0.60 |